plko 1 shctr vector Search Results


plko 1 shctr vector  (Addgene inc)
96
Addgene inc plko 1 shctr vector
Plko 1 Shctr Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 shctrl shplac8  (Addgene inc)
93
Addgene inc plko 1 shctrl shplac8
Plko 1 Shctrl Shplac8, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 shctrl  (Addgene inc)
96
Addgene inc plko 1 shctrl
Plko 1 Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 puro control shctrl  (Addgene inc)
96
Addgene inc plko 1 puro control shctrl
Plko 1 Puro Control Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 vector  (Addgene inc)
96
Addgene inc plko 1 vector
Plko 1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral vectors plko.1  (Shanghai GenePharma)
90
Shanghai GenePharma lentiviral vectors plko.1
Lentiviral Vectors Plko.1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 hygro lentiviral vector  (Addgene inc)
94
Addgene inc plko 1 hygro lentiviral vector
Plko 1 Hygro Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral vectors plko  (Addgene inc)
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Addgene inc lentiviral vectors plko
Lentiviral Vectors Plko, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 puro lentiviral vector  (Addgene inc)
95
Addgene inc plko 1 puro lentiviral vector
Plko 1 Puro Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 scramble shrna shctrl  (Addgene inc)
92
Addgene inc plko 1 scramble shrna shctrl
Plko 1 Scramble Shrna Shctrl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgfp c shlentil  (OriGene)
95
OriGene pgfp c shlentil
AGO2 regulates MYC mRNA transcript. (A) Relative mRNA expression of MYC mRNA of Huh1 and MHCC97H cells transduced with shCtrl and <t>shAGO2</t> lentivirus. (**p<0.005, Data are mean ± SEM). (B) Immunohistochemistry analyses of MYC and AGO2 in tumor xenograft tissues. (Images are at 200X magnification and scale bar is 20 μM). (C) RBP-RNA immunoprecipitation analysis. Anti-AGO2 or mouse anti-IgG were used to pull down RNAs cross-linked to AGO2 followed by qRT-PCR of MYC. Fold enrichment of transcript levels first adjusted to 2% input and normalized to IgG (**p<0.005, Data are mean ± SD of triplicates). (D) Relative luciferase (normalized to Renilla) activity of Huh1 and MHCC97H cells transduced with shAGO2 compared to shCtrl (**p<0.005, Data are mean ± SD of triplicates). (E) MYC mRNA transcript levels in shCtrl or shAGO2 after Actinomycin D (ActD) or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) treatment over specified time (Data are mean ± SEM).
Pgfp C Shlentil, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


AGO2 regulates MYC mRNA transcript. (A) Relative mRNA expression of MYC mRNA of Huh1 and MHCC97H cells transduced with shCtrl and shAGO2 lentivirus. (**p<0.005, Data are mean ± SEM). (B) Immunohistochemistry analyses of MYC and AGO2 in tumor xenograft tissues. (Images are at 200X magnification and scale bar is 20 μM). (C) RBP-RNA immunoprecipitation analysis. Anti-AGO2 or mouse anti-IgG were used to pull down RNAs cross-linked to AGO2 followed by qRT-PCR of MYC. Fold enrichment of transcript levels first adjusted to 2% input and normalized to IgG (**p<0.005, Data are mean ± SD of triplicates). (D) Relative luciferase (normalized to Renilla) activity of Huh1 and MHCC97H cells transduced with shAGO2 compared to shCtrl (**p<0.005, Data are mean ± SD of triplicates). (E) MYC mRNA transcript levels in shCtrl or shAGO2 after Actinomycin D (ActD) or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) treatment over specified time (Data are mean ± SEM).

Journal: Molecular cancer research : MCR

Article Title: AGO2 mediates MYC mRNA stability in hepatocellular carcinoma

doi: 10.1158/1541-7786.MCR-19-0805

Figure Lengend Snippet: AGO2 regulates MYC mRNA transcript. (A) Relative mRNA expression of MYC mRNA of Huh1 and MHCC97H cells transduced with shCtrl and shAGO2 lentivirus. (**p<0.005, Data are mean ± SEM). (B) Immunohistochemistry analyses of MYC and AGO2 in tumor xenograft tissues. (Images are at 200X magnification and scale bar is 20 μM). (C) RBP-RNA immunoprecipitation analysis. Anti-AGO2 or mouse anti-IgG were used to pull down RNAs cross-linked to AGO2 followed by qRT-PCR of MYC. Fold enrichment of transcript levels first adjusted to 2% input and normalized to IgG (**p<0.005, Data are mean ± SD of triplicates). (D) Relative luciferase (normalized to Renilla) activity of Huh1 and MHCC97H cells transduced with shAGO2 compared to shCtrl (**p<0.005, Data are mean ± SD of triplicates). (E) MYC mRNA transcript levels in shCtrl or shAGO2 after Actinomycin D (ActD) or 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) treatment over specified time (Data are mean ± SEM).

Article Snippet: HHT4 cells were cultured on fibronectin (BD Biosciences) coated plates. pLKO.1 shAGO2 and pLKO.1 shCtrl were purchased from OpenBiosystems (TRC lentivirus). pGFP-C-shlentil (shCtrl, shAGO2 1, and shAGO2 2) were purchased from Origene and packaged using HEK293T cells.

Techniques: Expressing, Transduction, Immunohistochemistry, Immunoprecipitation, Quantitative RT-PCR, Luciferase, Activity Assay

AGO2 stabilizes MYC transcript in Huh1 cells with impaired miRNA processing. (A) Representative immunoblot of Huh1.sgCtrl (Ctrl) and sg.DICER1 (DICER1−/−) cells. (B-C) Huh1 DICER1−/− cells express higher levels of MYC mRNA and lower Let-7 miRNA expression (**p<0.005, Data are mean ± SEM). (D) RNA immunoprecipitation of anti-AGO2 and anti-IgG in wildtype (Ctrl), DICER1−/− HCC cells (**p<0.005, Data are mean ± SEM). (E) MYC mRNA levels in Ctrl and DICER1−/− cells following Actinomycin D (ActD) treatment. (F) Ctrl and DICER1−/− cells were treated with shCtrl or shAGO2 lentivirus and MYC mRNA expression was measured (**p<0.005, Data are mean ± SEM).

Journal: Molecular cancer research : MCR

Article Title: AGO2 mediates MYC mRNA stability in hepatocellular carcinoma

doi: 10.1158/1541-7786.MCR-19-0805

Figure Lengend Snippet: AGO2 stabilizes MYC transcript in Huh1 cells with impaired miRNA processing. (A) Representative immunoblot of Huh1.sgCtrl (Ctrl) and sg.DICER1 (DICER1−/−) cells. (B-C) Huh1 DICER1−/− cells express higher levels of MYC mRNA and lower Let-7 miRNA expression (**p<0.005, Data are mean ± SEM). (D) RNA immunoprecipitation of anti-AGO2 and anti-IgG in wildtype (Ctrl), DICER1−/− HCC cells (**p<0.005, Data are mean ± SEM). (E) MYC mRNA levels in Ctrl and DICER1−/− cells following Actinomycin D (ActD) treatment. (F) Ctrl and DICER1−/− cells were treated with shCtrl or shAGO2 lentivirus and MYC mRNA expression was measured (**p<0.005, Data are mean ± SEM).

Article Snippet: HHT4 cells were cultured on fibronectin (BD Biosciences) coated plates. pLKO.1 shAGO2 and pLKO.1 shCtrl were purchased from OpenBiosystems (TRC lentivirus). pGFP-C-shlentil (shCtrl, shAGO2 1, and shAGO2 2) were purchased from Origene and packaged using HEK293T cells.

Techniques: Western Blot, Expressing, Immunoprecipitation